Anigen Rapid AIV Ag Test Kit

Features

Anigen Rapid AIV Ag Test Kit(RG15-01) is a solid phase immune chromatographic assay for the qualitative detection of all subtypes(H1 ~ H16) of Avian Influenza Virus Type A Antigen in avian cloacae, tracheal and feces swabs.

Introduction

Influenza viruses that infect birds are called “Avian Influenza virus(AIV)” with only influenza type A viruses infecting birds. Influenza type A viruses are divided into subtypes based on two proteins, hemagglutinin(HA) and neuraminidase(NA), on the surface of the virus. AIV can infect chickens, turkeys, pheasants, quail, ducks, geese, and guinea fowl, as well as a wide variety of other birds. AIV is spread primarily through direct contact from infected birds to healthy birds, and through indirect contact with contaminated equipment and materials. The virus is excreted through infected birds’ feces and secretions from their noses, mouths and eyes.

Specifications

• Principle : Immune chromatographic assay using Direct Sandwich Method
  • Monoclonal anti-AIV(Capture) – AIV Type A – Monoclonal anti-AIV(Detector)
  • Monoclonal antibody against Type A specific nucleoprotein of Avian influenza virus
• Purpose : Detection of Avian influenza type A virus(H1~H16)
• Specimen : Avian cloaca swab or feces
• Reading time : Within 15 minutes
• Sensitivity : 100% by flock (n=19), 77.3% by feces(n=150)
• Specificity : 100% vs. HA, PCR (n=1,402)
• No cross reaction against NDV, IBV, ILTV, Pneumovirus, Reovirus, IBDV, MDV,
• mycoplasma and REV
• Minimum Detection Limit : 10^3.1 EID₅₀/ml
• Shelf life : 26 months
• Storage temperature : 2~30°C
• Packing size : 25 individual devices/Kit
• The Pigeons have very low possibility of AIV infection and the specimens from pigeon
• may show unusual result in the test kit
• Cat. No. : RG15-01

Characteristics

• Optimal screening method of AIV in the farm or laboratory
• Detection of all avian influenza type A viruses(H1N1~H16N9)
• High Sensitivity compared with PCR, virus isolation method, HA and other immunoassay
• High specificity : No cross-reaction with other avian viruses.
• Detection of AIV in Chickens, Ducks, Turkeys, and Geese
• World’s first commercialized rapid test kit for detection of avian influenza virus since December 2003
• Easy assay procedure : No technical expertise required, and Saving Labor&Time
• Rapid test result : Within 15 minutes
• No additional equipment is required
• It is recommended to test 10 birds in every AIV suspected flock for the excellent result.
• Test result with trachea and cloacal swab shows more reliable result than feces
• NVRQS/Ministry of Agriculture & Forestry of Korean approved

Test Procedures

1. Collect a cloaca or tracheal or fecal swabs
2. Dilute swab sample with assay diluent and add 4 drops on a sample hole of the test device
3. Wait for 15 minutes and read the test result